ABSTRACT
<p><b>OBJECTIVE</b>To explore the signal transduction pathway mediated by thrombopoietin (TPO) in the inflammation model of microglia induced by lipopolysaccharide (LPS).</p><p><b>METHODS</b>The inflammation model of microglia BV2 cells was prepared by LPS of 0.5 and 1.0 μg/mL stimulation. The expression of TPO and ERK mRNA in BV2 cells was detected by real time quantitative PCR. Western blot was used to evaluate the expression of TPO and ERK protein in BV2 cells. TPO and IL-6 contents in the culture supernatant fluid were measured using ELISA.</p><p><b>RESULTS</b>LPS stimulation increased significantly the mRNA and protein expression of TPO and ERK in BV2 cells, especially at the concentration of 1.0 μg/mL for 12 hrs stimulation. There was a significant positive correlation between the mRNA and protein expression of TPO and ERK.</p><p><b>CONCLUSIONS</b>Signal transduction pathway of ERK1/2 participates in the activation of TPO in inflammatory injury of BV2 cells.</p>
Subject(s)
Animals , Mice , Enzyme-Linked Immunosorbent Assay , Extracellular Signal-Regulated MAP Kinases , Genetics , Metabolism , Inflammation , Microglia , Pathology , RNA, Messenger , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction , Physiology , Thrombopoietin , Genetics , PhysiologyABSTRACT
<p><b>OBJECTIVE</b>To explore the neuro-protective effect and mechanism of qingkailing injection (QKL) against cerebral injury caused by E. coli-meningitis (CM).</p><p><b>METHODS</b>The CM model rabbits were treated by ampicillin with QKL as adjuvant. The leukocyte count and protein content in cerebral spinal fluid (CSF), the contents of water, sodium, potassium and calcium in cerebral tissues were measured before, 16 h and 26 h after Bacillus coli injection respectively. The expression of matrix metalloproteinase-9 (MMP-9) was determined at the same time.</p><p><b>RESULTS</b>Adjunctive treatment with QKL can not only inhibit the increase of leukocyte cells, protein content in CSF, and water, sodium, calcium content in cerebral tissues, but also the decrease of potassium content revealed during simple antibiotic treatment. It also can decrease the expression of MMP-9 in cerebral tissues of rabbits with CM.</p><p><b>CONCLUSION</b>As an adjunctive treatment, QKL can prevent transient inflammatory reaction and aggravation of brain injury in CM induced by simple antibiotic treatment, its mechanisms might relate with calcium antagonism and attenuation of MMP-9 expression in brain tissues.</p>